Keywords : IFN


Evaluation the Levels of IFN-Gamma , IL-10 and Concentration of Zn in Children with Visceral Leishmaniasis

Mais N. Kamil; Sabah Al-Najar; Nahla Ghanim

Iraqi Postgraduate Medical Journal, 2013, Volume 12, Issue 4, Pages 504-510

ABSTRACT:
BACKGROUND:
Visceral Leishmaniasis (VL) is a systemic infection of the reticulo- endothelial system that could affect on immune system and biochemical parameters like the concentration of trace elements which may be significantly changed .
OBJECTIVE:
Evaluating the level of cytokines (INF- γ ,IL-10) and concentration of Zn in Visceral Leishmaniasis in children after diagnosis .
PATIENTS AND METHODS:
A total of (98) child their ages rang (6m-5y) were attending to the Central Public Health Laboratory and Teaching Laboratories of Medical City suspected to be infected with kala-azar, the diagnosis was done by both IFAT technique and Rapid Kala-azar (r-K39) detecting test and evaluating the level of cytokines (INF- γ, IL-10) by ELISA and concentration of trace element ( Zn ) by Atomic Absorption Technique .
RESULTS :
The dipstick test ( r-K39) showed a high sensitivity of (92.1%) compared to IFAT (73.6%) with a specificity of 100% for both tests. Serum samples of 56 child with positive results in IFAT and rK39 test were used for the investigation of IL-10, IFN-γ. The mean levels of IL-10 (80.207±77.54 Pg/ml) and IFN- γ (5.426±4.599 IU/ml) were highly significant increased in patients compared to healthy controls .The mean level of serum Zn (69.71±7.97μg/dl) was significantly low in VL patients compared to healthy controls .
CONCLUSION :
This study showed that the ( r-K39) dipstick test could be more sensitive than IFAT technique in the diagnosis of VL with a specificity of 100 % for both test according to clinical diagnosis. Both IFN-γ & IL-10 were significantly increased in VL patients as compared to controls group. The concentration of Zinc was significantly lower in VL patients than healthy controls.

VCAM-1 is Another Ugly Face of IFN-γ in Women with Recurrent Spontaneous Abortion

Asmaa Baqer Al-Obaidi; Manal Adnan Habib; Suhad Mohmamad

Iraqi Postgraduate Medical Journal, 2011, Volume 10, Issue 2, Pages 229-236

ABSTRACT:
BACKGROUND:
The Th-1 cytokines were well implicated in adversely affecting pregnancy and the mechanism by which they cause pregnancy loss was suggested to be mainly vascular by activation of mediators of inflammation like certain adhesion molecules.
OBJECTIVE:
To study the pathological effect of IFN-γ and VCAM-1 in recurrent pregnancy loss.
PATIENTS AND METHODS:
The study included three groups of women; Group A: patients had recurrent abortion (n=24), Group B: patients had spontaneous abortion for the first time (n=10), Group C: women with elective pregnancy termination (n=6). Curate samples obtained from these women were subjected for in situ hybridization technique to determine the in situ expression of IFN-γ, and immunohistochemistry analysis to detect the expression of VCAM-1.
RESULTS:
The in situ expression of IFN-γ was significantly higher in women with RSA as compared with normal pregnant and first abortion groups (p=0.000 and 0.002) respectively, and the expression of VCAM-1 was also significantly higher (p=0.005) in women with RSA as compared with those who had abortion for the first time, with a positive correlation between the expression of IFN-γ and VCAM-1 (r=0.418; p<0.05) in women with RSA.
CONCLUSION:
The data of this study strengthen the possibility that type-1 immune response may have the upper hand in the pathology of RSA on multidirectional bases including up regulation of the surface expression of VCAM-1