Keywords : her
Immunohistochemical Expression of Her2/Neu Receptor in Human Colorectal Carcinoma (A Clinicopathological Study)
Iraqi Postgraduate Medical Journal,
2014, Volume 13, Issue 3, Pages 424-429
Colorectal adenocarcinoma is the most common type of gastrointestinal cancers in Iraq, and according to the Iraqi cancer registry (ICR) reports, the incidence of colorectal carcinoma was 2.99% of whole body malignancies (ICR 2010). In males, it’s the 5th common cancer while in females it’s the 4th most common cancer.
Her2/neu is an important oncogene in breast cancer, but its prevalence and significance in colorectal carcinoma have been documented.
To determine the frequency and the pattern of Her2/neu expression in colorectal adenocarcinoma by immunohistochemical technique and to correlate this expression with different clinicopathological parameters.
MATERIAL AND METHOD:
Twenty five cases of colorectal adenocarcinoma were studied, these cases were diagnosed in private laboratories in Baghdad/Iraq from November 2011 to march 2013. Clinicopathological parameters such as age, gender, pathological diagnosis, including the tumor site, size, lymph nodes status, grade and stage of tumor were taken from patients file. Sections of 4 µm stained by H&E stain and immunohistochemical stained for Her2/neu.
Using infiltrative ductal carcinoma of the breast as control positive, evaluation of Her2/neu expression by immunohistochemistry in all cases was performed.
Fourteen (56%) of the cases were males, 11 (44%) case were females, with age distribution ranging from (24-89) years, with a mean age of 56.5 years. Tumor size ranges between 2.5-10 cm, with mean of 6.25 cm. Seven (28%) cases were localized in the cecum, 5 (20%) from each rectum, sigmoid and left colon, respectively and 3 (12%) involving more than one segment of the colon.
Histologically the tumor grade ranges from moderately differentiated in 23 (92%) cases and poorly differentiated in 2 (8%) cases. Regarding pathological staging (TNM system), 5 (20%) were stage T2, 17 (68%) were stage T3 and 3 (12%) were T4. Lymph node involvement found in 10(40%) of the cases and distant metastasis was found in 2 (12%) cases. Her2/neu expression was present in 4(16%) cases of 25 colorectal adenocarcinoma; there was no correlation with age, sex, histopathological grade, location, lymph nodes status and tumor invasion.
Concerning data exists about the prevalence of her2/new expression in colorectal adenocarcinoma, there was no significant correlation between her2/neu over expression and tumor size, grade, localization of the primary tumor, lymph nodes status and depth of invasion.
Iraqi Postgraduate Medical Journal,
2014, Volume 13, Issue 2, Pages 268-272
Overexpression of Human epidermal growth factor receptor 2(Her2/neu)
Protein has been observed in many human cancers including gastric cancer . Gastric cancer remains the most common cause of cancer –related death worldwide. Her2/neu overexpression
in gastric cancer associated with poor prognosis and shorter survival rate , the assessment of this protein for selection of eligible patients for treatment with trastzumab in addition to conventional chemo therapy.
To evaluate the Her2/neu over expression in gastric cancer by immunohistochemical
Technique and to find the correlation between the protein exopression and clinicopathological
Parameters as age ,sex, histological types and grade of the tumor.
MATERIALS AND METHODS :
This is a pro-& retrospective study conducted at the pathology department of Al-Jamhuri Teaching hospital and some private laboratories . A total of 30 cases of gastric cancer were collected and diagnosed in the period spanning from April 2010 to April 2012, .An immunohistochemical technique was used for the assessment of Her2 against age,sex, histological types and grade of tumor
The mean age of the patients was 54.5 years ranging from 30 to 87 years, Her2/neu overexpression was shown in 30% of the cases , in this study there is no relation between Her2 positivity and age or sex, , it was associated with histological types p-value = 0.04,where as 42.6 % of the intestinal type show immunoreactivity and 11.1% of diffuse type,while two cases of mixed types were shown no reactivity(0.0% ). Her2 positivity was associated with moderate differentiated tumor (50%) more than poorly differentiated (20%) but not reaching the statistical significance.
Her2/neu positivity was found in 30% of gastric cancer , there is no relation between the age or sex of the patient & Her2 positivity while intestinal type and moderately differentiated tumor were associated with high percentage of Her2 positivity.