Keywords : Oxidative stress


LDL Particles Size Index and Lipid Peroxidation in Type 2 Diabetic Male Patients

Kismat M.Turki; Hind Sh.Ahmed; Perry H.Saifullah

Iraqi Postgraduate Medical Journal, 2013, Volume 12, Issue 1, Pages 57-61

ABSTRACT:
BACKGROUND:
Diabetes mellitus is a heterogeneous condition reflecting different metabolic disorders accompanied by a variety of complications. In DM, there is a change in oxidative stress (elevation in free radical generation with increase lipid peroxidation and derived oxidized products, and serum lipid profile abnormalities.
OBJECTIVE:
Several free radical species are normally produced in the body to perform specific functions. Increased free radicals in diabetes may cause the pathogenesis of atherosclerosis, and the degenerative disorders. In the present study, the oxidative stress in type 2 diabetic male patients was evaluated by estimating the lipid peroxidation. Malondialdehyde (MDA) is one of the major aldehyde derived from lipid peroxidation.
PATIENTS AND METHODS:
Serum MDA, Oxidized HDL (ox.HDL), and lipid profile were measured after 12 hr fasting in 30 diabetic patients, their age range was (40-55) years and compared with 30 healthy controls. RESULTS:
Serum MDA and ox.HDL were significantly increased in the diabetic group (P<0.05). All patients had significant elevation in serum levels of glycated hemoglobin (HbA1c), total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C), and LDL particles size index (TG/HDL-C ratio).
CONCLUSION:
This may be due to different glycemic control in these patients. There was a significant positive correlation between serum MDA and LDLs size index, while serum MDA was negatively correlated with serum HDL-C in diabetic patients. Our results indicate that oxidative stress status increases during type 2 diabetes mellitus in parallel to glucose and lipid changes

Analysis of DNA Damage and Oxidative Stress in Human Spermatozoa and Some Biochemical Changes in Seminal Plasma and their Correlation with Semen Quality of Infertile Men

Ghassan T. Alani; Sermed S. Khonda; Hedef D. El Yaseen

Iraqi Postgraduate Medical Journal, 2011, Volume 10, Issue 1, Pages 81-88

ABSTRACT:
BACKGROUND:
Numerous studies have shown the presence of DNA strand breaks in human ejaculated spermatozoa. The nature of this nuclear anomaly and its relationship to patient etiology is however poorly understood.
OBJECTIVE:
This study was done to investigate the relationship between nuclear DNA damage, assessed using the TUNEL assay and a number of biochemical parameters including zinc level, copper level, and the malondialdehyde (MDA) level as an indicator of the lipid peroxidation and also the standard general seminal fluid examination.
MATERIAL AND METHODS:
In this study 100 seminal samples were tested. Seventy five infertile patients aged (30.33±5.96) and 25 normal people aged (29.84±6.434) were included as a control. All tests were done to all the samples except the DNA fragmentation which was done to 60 samples only (45 patients and 15 normal).
RESULTS:
A positive relationship between DNA damage level and infertility was found. Also there were higher levels of MDA level and DNA damage in spermatozoa of infertile patients (P=0.001). We found also absence of difference in the copper level between the two groups. Regarding seminal zinc level we found that there was a low zinc level in infertile patients (P=0.001). Non-significant correlations between zinc level and the percentage of DNA fragmentation and the MDA levels were obtained.
CONCLUSION:
This study suggested that sperm DNA analysis might be better discriminator between infertile and fertile men than standard semen analysis also oxidative stress and sperm DNA damage contributed to sperms dysfunction. The infertile samples associated with high levels of oxidative stress and low seminal zinc levels.

The Role of Oxidative Stress in the Pathogenesis of Systemic Lupus Erythmatosus (SLE) Among Some Iraqi Patients

Ihab Ibrahim AbdulWahab

Iraqi Postgraduate Medical Journal, 2007, Volume 6, Issue 4, Pages 286-288

ABSTRACT:
BACKGROUND AND OBJECTIVE:
Systemic Lupus Erythematosus (SLE) is a chronic progressive autoimmune disorder with a wide spectrum of clinical and immunological abnormalities, predominantly developed in women of childbearing age, oxidative stress has been postulated in many pathological conditions including atherosclerosis , inflammatory conditions and some autoimmune disease such as multiple sclerosis , rheumatoid arthritis and Systemic Lupus Erythematosus (SLE).
The present study was conducted to support the idea of oxidative stress
during the pathogenesis of (SLE) among some Iraqi patients, comparing it with healthy controls group matched for the same sex and age .
PATIENTS AND METHODS:
Twenty-three Iraqi Arab patients (21 females and 2 males) with Systemic Lupus Erythmatosus (SLE) admitted to Baghdad Teaching Hospital were included in the present study ,and have been compared with 13 healthy controls .
The patients group were diagnosed as having SLE according to the basis of the revised criteria of the American College of Rheumatology ,
Analysis of the parameters of oxidative stress , serum malondialdehyde (MDA) and glutathion (GSH) was performed in all patients before starting any type of drug treatment and their levels were compared with those belong to healthy controls.
RESULTS:
The results presented in this study showed elevated serum MDA concentration in the SLE patients group compared to healthy control ; however , this elevation failed to reach the statistically significant (P<0.05).
Significant variation was also demonstrated in serum levels of glutathione between both patients group , in which serum GSH level was significantly lower in the diseased group compared to healthy controls ( P<0.05).
CONCLUSION:
Oxidative stress mechanism can be proposed as a cause and / or consequence in the pathogenesis of SLE , supporting the theory of free radical –induced tissue damage in this respect.

Effects of Exposure Duration to Liquefied Propane on Lipid Peroxidation and Antioxidant Enzymes in Gas Workers

Nada Khadum Al- Tae

Iraqi Postgraduate Medical Journal, 2007, Volume 6, Issue 1, Pages 76-79

ABSTRACT:
BACKGROUND:
Toxic environmental agents include a host of chemicals and pollutants that may available in tobacco smoke and tar, the atmosphere, drugs, the work place, the food and water supply and from radiation and infectious organisms. These agents may exert multiple types of harmful effects on human body, including lipid peroxidation.
OBJECTIVES:
To assess the effects of duration of exposure on lipid peroxidation and antioxidant enzymes in liquefied propane gas workers.
METHODS:
Fifty five adult male gas workers (age mean 36. 6 ± 3.8 years) in liquefied propane gas stations in the District of Baghdad were enrolled in the present study. They were allocated into 3 groups according to the duration of exposure to LPG (1-9, 10-20 and more than 20 years). Twenty five healthy subjects, not exposed to LPG, with age mean comparable to that of workers (37.2 ± 4.0 years) were utilized as controls.
RESULTS:
The results showed significant differences in the levels of MetHb, lipid peroxidation parameters and antioxidant enzymes activities in LPG workers compared to controls. Meanwhile, workers with different duration of exposure to LPG demonstrated significant differences only in MetHb, MDA and catalase activity.
CONCLUSION:
Workers with different duration of exposure to LPG demonstrated significant differences only in MetHb, MDA and catalase activity. In conclusion, the changes in lipid peroxidation and antioxidant enzymes may be useful as indicator for the impact of duration of exposure in LPG workers

Evidence of Correlation between Some Auto Antibodies with Complement Component in Lupus Nephritis

Nawar Abass Abud Noor; Kholod Daher Habib Al-Shemari; Lilyan W. Sersam; Ibtesam Ghadhban Auda; Nada Khadum Al- Tae; Aroub Abdul Rahman Al-Kaisi

Iraqi Postgraduate Medical Journal, 2007, Volume 6, Issue 1, Pages 54-57

ABSTRACT:
BACKGROUND:
lupus nephritis is a fatal complication of systemic lupus erythematosus (SLE), the serological hallmark of SLE is the presence of circulating autoantibodies directed against a wide variety of nuclear, cytoplasmic and plasma membrane antigens among which are anticardiolipin (ACL),anti-B2glycoprotein and anti-C1q antibodies, together with the consumption of complement component (C3 &C4) by immune complex mediated reaction.
AIM OF STUDY:
To shed light on the relations among these autoantibodies and with complement component in lupus nephritis patients.
METHODS:
Tthe study was conducted on 25 patients with lupus nephritis, attended the renal clinic in specialized surgical hospital/ medical city /Baghdad, 25 lupus patients without nephritia and 25 healthy controls.
Enzyme linked immunsorbant assay was used for detection for ACL, anti-B2GP, anti-C1q, while single radial immundiffusion plates used for the estimation of C3& C4.
RESULTS AND CONCLUSION:
Anticardiolipin antibodies correlated significantly with anti-B2GP IgG & IgM (P<0.05), anti-C1q antibodies (P

Keywords

Autoantibodies
---
C3
---
C4 complement
---
lupus nephritis.