Keywords : immunophenotyping

Immunophenotyping of Bone Marrow Biopsies in 26 Patients with Non-Hodgkin's Lymphoma, Using Anti-CD3, CD8, CD19 and CD20 Monoclonal Antibodies

Faris. H. Jaafer; Raad Jaber Musa M

Iraqi Postgraduate Medical Journal, 2014, Volume 13, Issue 3, Pages 430-433

Immunophenotyping with monoclonal antibodies (MoAbs) directed against lymphoid-associated antigens, immunohistochemical staining on paraffin-embedded BM biopsy material, and molecular studies of Ig genes/T-cell receptor genes or lymphoma-associated gene translocations should be used in the global approach to the patient with malignant lymphoma.
1. To determine the subtypes of non-Hodgkin lymphomas (B- or T cell) in the bone marrow using anti-CD3, CD8 monoclonal antibodies for T-cell and anti-CD19 and CD20 for B-cells.
2. Correlation of the subtypes of NHL (B- or T cell) with the morphology and pattern of bone marrow infiltration.
A retrospective study, done in Al-Kadhymia teaching hospital during the period from 1/10/2010 to1/2/2011.The study consisted 26 adult patients, who were diagnosed as Non-Hodgkin lymphomas by undergoing a BM biopsy. Immunohistochemical staining of the paraffin-embedded sections of BM trephine biopsies was performed in all cases and used standard techniques with monoclonal anti-CD8, CD20 and dual immunofluorscence-labelled CD3, and CD19 antibodies and also all stained with Hematoxylin and Eosin (H&E) for morphologic assessment.
The 26 cases of NHL comprised of 14 male (54%) and 12 female patients (46%) . The median age was (57.32) year ranged from 27-85 years. There were 23 cases of B-cell cases (88.5%) and 3 cases of T-cell lineage (11.5%) of all the cases. Among all the B-cell lymphomas, 15 cases showed interstitial infiltration in the bone marrow, while among the T-cell lymphoma two cases showed diffuse infiltration.
1. In these 26 cases NHL patients with marrow involvement, B cell phenotype comprised 88% of cases.
2. B-cell NHLs had predominance of interstitial infiltration in bone marrow biopsies in comparison with the T-cell lymphoma, in which diffuse infiltration was predominant.

Activated Peripheral Blood Lymphocyte Subpopulation in Newly Diagnosed Type 1 Diabetes Mellitus Children

Eman Mahdi Saleh

Iraqi Postgraduate Medical Journal, 2008, Volume 7, Issue 2, Pages 112-120

In Type 1 Diabetes Mellitus (T1DM), numerous changes in the cellular as well humoral immune response have been identified. However, it is not known whether both the CD4+ and CD8+ subpopulation or only one of these or CD19+contains increased numbers of activated cells.
The aim was to study the activated lymphocyte subpopulation by use of monoclonal antibodies to T-cell and B-cell antigens which is known to be expressed on activated cells.
A total of 60 T1DM patients who had newly onset of the disease (diagnosed was from one week up to five months) were included in the present study, all the patients were treated with daily replacement doses of insulin. Fifty apparently healthy control subjects underwent the PBL phenotyping. Phenotyping of surface antigens was done by direct Immunoflurocent (IFT) technique using mouse antihuman CD3, CD4, CD8, CD45RA, CD19, and activated markers CD45RO, DR-antigen and CD38.
T1DM patients showed a remarkable lowering in CD3+, CD8+, and CD45RA+ cells (p<0.0001), but the decrease in CD4+ cells percentage was not significant. In contrast, a significant elevation of activation markers includes (CD45RO+, HLA-DR+ and CD38+ cells) were observed in patients in addition to a significant increase of CD19+ cell percentage and CD4+: CD8+ ratio in the patients.
This study provides evidence that abnormalities of T-cells regulation are detectable in patients with T1DM.