Study of H.Pylori in a Group of Iraqi Patients with Inflammatory Bowel Disease (Histological and Molecular Study)
Iraqi Postgraduate Medical Journal,
2012, Volume 11, Issue 0, Pages 734-741
Helicobacter pylori is one of the commonest bacterial pathogens in human. The organism is associated with development of peptic ulcer diseases, lymphoproliferative disorders and gastric cancer.
Helicobacter pylori could be isolated from patients with inflammatory bowel disease (IBD) but still the possibility of etiological link need further study therefore
Is to assess the possible causal factor of H.pylori in development of inflammatory bowel disease, in addition to assess Cytotoxic associated gene A(CagA) gene status in H.pylori positive samples.
PATIENTS AND METHODS:
Study involved 120 patients of colonoscopically determined normal colon (n=90) & patients with IBD (n=30) including both Ulcerative colitis(n=16) and Crohn’s disease(n=14). Those patients is further divided into three age groups including <20 years group, 20-40 years group and >40 years group of Iraqi patients. Endoscopic specimens after histopathological confirmation of diagnosis will be tested for Biopsy Urease Test (BUT) and Hematoxylin & Eosin (H&E) methods (for detection of H. pylori) also detection of CagA mRNA using In Situ Hybridization technique with a biotin labeled probe (to specify pathogenic H. pylori)
H.pylori detected in 36.7% (using BUT) and 30% (using H&E) in the colon of IBD patients and 33.3% (using BUT) and 26.7% (using H&E) in patients with normal colon (NC patients) as a control. Among our H.pylori positive patients, there was significant difference (P=0.036) regarding CagA status in which 25% were CagA positive of NC patients and 66.7% CagA positive H.pylori in IBD patients using In Situ Hybridization technique.
H.pylori was isolated from nearby or the site of lesion of patients with inflammatory bowel disease although there was no statistical relationship between H.pylori and IBD. In addition CagA genes were more prominent in H.pylori that isolated from IBD patients than H.pylori of normal colon. Also there was no possible relationship between age and infection rate of H.pylori in both IBD & NC patient
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