Ethical consent: all manuscripts reporting the results of experimental investigations involving human subjects should include a statement confirming that  informed consent was obtained from each subjects or subject’s guardian ,after approval of the experimental protocol by a local human ethics committee .ethical approval is necessary not only for patient consent ,but to avoid duplication of work from the same institute, and confirms that the institute gives approval to release the data .when reporting experiments on animals ,authors should indicate whether the institutional  and national guide for the care and use of laboratory animals was followed.